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whole-genome dna microarray (8 × 15k format  (Agilent technologies)


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    Agilent technologies whole-genome dna microarray (8 × 15k format
    Whole Genome Dna Microarray (8 × 15k Format, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Microarray results obtained by hybridization of seven cloned genes encoding different CAZymes from diverse microorganisms targeted by the FibroChip.

    Journal: Frontiers in Microbiology

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota

    doi: 10.3389/fmicb.2018.00215

    Figure Lengend Snippet: Microarray results obtained by hybridization of seven cloned genes encoding different CAZymes from diverse microorganisms targeted by the FibroChip.

    Article Snippet: Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling.

    Techniques: Microarray, Hybridization, Clone Assay

    Microarray results obtained after hybridization of genomic DNA from selected bacterial strains.

    Journal: Frontiers in Microbiology

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota

    doi: 10.3389/fmicb.2018.00215

    Figure Lengend Snippet: Microarray results obtained after hybridization of genomic DNA from selected bacterial strains.

    Article Snippet: Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling.

    Techniques: Microarray, Hybridization

    Microarray gene expression analysis of the targeted genes from Fibrobacter succinogenes S85 cultivated with cellobiose (Clb) or wheat straw (WS) as a sole substrate. Relative expression of expressed genes on WS versus Clb is expressed as log2 ratio (WS/Clb) ± SEM. Genes are denominated by the accession number of the protein encoded.

    Journal: Frontiers in Microbiology

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota

    doi: 10.3389/fmicb.2018.00215

    Figure Lengend Snippet: Microarray gene expression analysis of the targeted genes from Fibrobacter succinogenes S85 cultivated with cellobiose (Clb) or wheat straw (WS) as a sole substrate. Relative expression of expressed genes on WS versus Clb is expressed as log2 ratio (WS/Clb) ± SEM. Genes are denominated by the accession number of the protein encoded.

    Article Snippet: Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling.

    Techniques: Microarray, Expressing

    Bacterial strains, plasmids, and primers used in this study

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection ▿

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Bacterial strains, plasmids, and primers used in this study

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Plasmid Preparation, Sequencing, Mutagenesis, Real-time Polymerase Chain Reaction

    Growth of APEC O1 in LB and chicken serum. Optical density (OD600) (A) or CFU counts (B) were used to assess the growth of APEC O1 in culture medium LB (□) and chicken serum (▪). APEC O1 grew faster in serum than in LB, suggesting that the APEC O1 can rapidly adapt to chicken serum.

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection ▿

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Growth of APEC O1 in LB and chicken serum. Optical density (OD600) (A) or CFU counts (B) were used to assess the growth of APEC O1 in culture medium LB (□) and chicken serum (▪). APEC O1 grew faster in serum than in LB, suggesting that the APEC O1 can rapidly adapt to chicken serum.

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques:

    Heat map visualizing regulated genes in APEC O1 grown in chicken serum compared to growth in LB. Genes found to be significantly regulated are indicated by either green (upregulation) or red (downregulation). Significantly differentially expressed genes of APEC O1 during growth in serum compared to that in LB are listed in columns 2 and 3. The genes are sorted by functional categories (column 1) according to annotation by the J. Craig Venter Institute.

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection ▿

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Heat map visualizing regulated genes in APEC O1 grown in chicken serum compared to growth in LB. Genes found to be significantly regulated are indicated by either green (upregulation) or red (downregulation). Significantly differentially expressed genes of APEC O1 during growth in serum compared to that in LB are listed in columns 2 and 3. The genes are sorted by functional categories (column 1) according to annotation by the J. Craig Venter Institute.

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Functional Assay

    Significantly upregulated genes of APEC O1 localized in genomic islands that are not present in E. coli K-12

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection ▿

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Significantly upregulated genes of APEC O1 localized in genomic islands that are not present in E. coli K-12

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Expressing, Binding Assay

    Significantly upregulated genes from plasmids of APEC O1 cultured in chicken serum compared to results with LB

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection ▿

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Significantly upregulated genes from plasmids of APEC O1 cultured in chicken serum compared to results with LB

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Cell Culture, Expressing, Plasmid Preparation

    Comparison of gene regulation analyzed by microarray (filled) or real-time quantitative RT-PCR (open). Real-time quantitative RT-PCR was used to validate the expression level for 11 selected genes, including 6 upregulated genes and 4 downregulated genes revealed by microarray analysis.

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection ▿

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Comparison of gene regulation analyzed by microarray (filled) or real-time quantitative RT-PCR (open). Real-time quantitative RT-PCR was used to validate the expression level for 11 selected genes, including 6 upregulated genes and 4 downregulated genes revealed by microarray analysis.

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Comparison, Microarray, Quantitative RT-PCR, Expressing